EMMAWiki/WebDocumentation/GeneralUsage/ImportDatafromArrayLims: Difference between revisions
imported>ThassoGriebel No edit summary |
imported>ThassoGriebel No edit summary |
||
| Line 15: | Line 15: | ||
== Step 2 Select Slides == | == Step 2 Select Slides == | ||
After the project selection is done you have to select the slides you want to import. | After the project selection is done you have to select the slides you want to import. The list shows all slides available in the selected project and lists there slide names and optionally a type and some remarkes. To be listed here the slides also have to contain valid images to be importable. Use the checkboxes to select one or more slides and continue with step 3. | ||
== Step 3 Image Selection | |||
Here you have to select appropriate images from the selected slides and assign them to the channels. There are two channels, one for the "Experiment" (the first channel) and one for "Control" (the second channel). Each [[ArrayLims]] Slide contains two images and now you have to assign the images to the channels, that is, choose one image for channel one and the other image for channel two. The [[ArrayLims]] System tells you wich color marker used for which image (e.g. cy5 and cy3), and as long as you know what you did, you should be able to assigne the correct images to the channels, just be careful not to switch channels if you don't want to. After the image to channel assignment you can contiue to the next step, but don't be confused, it will take a while until the "Step 4" page is loaded, because we have to parse the appropriate data files and count the spot information which takes some time because the files are quite large. | |||
== Step 4 Choose [[ArrayLayout]] == | |||
After the data file parsing is done, you have to select a proper [[ArrayLayout]] for each slide. In the normal case there will just be one Layout listed and you just have to select this one. If the list contains more than one layout for a slide, you have to select the correct one used for your Experiment. If you try to import slides where EMMA does not list any layouts, you have to import the appropriate layout first before you can import the [[ArrayLims]] slides according to the layout. | |||
== Step 5 Selection Overview == | |||
Finally choose a name for the selected arrays and take a look at the overview shown on this page | |||
to verify that all data are correct before you start the import.If you find a mistake in the selected data, please use your browsers back button | |||
and fix it, otherwise click the "Start Import" Button to submit the import job. | |||
== Step 6 Job Submitted == | |||
This is just an information page that tells you that the import job was successfully submitted and is running now. To view the status of the job, you can use the [[ViewResults]] page reachable from the [[BasicMenu]]. | |||
Revision as of 13:47, 18 April 2005
Import Data from ArrayLims
This page describes how to import data from ArrayLims to the EMMA System. To be able to import data to EMMA one has to select an Experiment first. All imported Arrays will be appended to the selected Experiment. After selecting an Experiment, there is a "Import from ArrayLims" link in the Basic Menu. Use this link to get to the main import page.
The ArrayLims import is devided into 6 steps.
Step 1 Select an ArrayLims Project
Step one is the selection of an appropriate ArrayLims Project. The selection box lists all accessible ArrayLims Projects. Now have to select the one you want to import arrays from.
Step 2 Select Slides
After the project selection is done you have to select the slides you want to import. The list shows all slides available in the selected project and lists there slide names and optionally a type and some remarkes. To be listed here the slides also have to contain valid images to be importable. Use the checkboxes to select one or more slides and continue with step 3.
== Step 3 Image Selection Here you have to select appropriate images from the selected slides and assign them to the channels. There are two channels, one for the "Experiment" (the first channel) and one for "Control" (the second channel). Each ArrayLims Slide contains two images and now you have to assign the images to the channels, that is, choose one image for channel one and the other image for channel two. The ArrayLims System tells you wich color marker used for which image (e.g. cy5 and cy3), and as long as you know what you did, you should be able to assigne the correct images to the channels, just be careful not to switch channels if you don't want to. After the image to channel assignment you can contiue to the next step, but don't be confused, it will take a while until the "Step 4" page is loaded, because we have to parse the appropriate data files and count the spot information which takes some time because the files are quite large.
Step 4 Choose ArrayLayout
After the data file parsing is done, you have to select a proper ArrayLayout for each slide. In the normal case there will just be one Layout listed and you just have to select this one. If the list contains more than one layout for a slide, you have to select the correct one used for your Experiment. If you try to import slides where EMMA does not list any layouts, you have to import the appropriate layout first before you can import the ArrayLims slides according to the layout.
Step 5 Selection Overview
Finally choose a name for the selected arrays and take a look at the overview shown on this page to verify that all data are correct before you start the import.If you find a mistake in the selected data, please use your browsers back button and fix it, otherwise click the "Start Import" Button to submit the import job.
Step 6 Job Submitted
This is just an information page that tells you that the import job was successfully submitted and is running now. To view the status of the job, you can use the ViewResults page reachable from the BasicMenu.