RNA: Difference between revisions

From BRF-Software
Jump to navigation Jump to search
imported>TimKahlke
No edit summary
m (5 revisions)
 
(3 intermediate revisions by one other user not shown)
Line 1: Line 1:
__NOTOC__
__NOTOC__
This page is for submitting the data of your RNA production. Fill out ''' every '''  field marked with a red star ( ''' required fields ''' ). If you just want to enter the data of '''one''' RNA do '''not''' fill '''any''' of the fields of '''RNA2'''. An error message will appear on top of the page if you submitted data in the wrong format or did not fill all reqired fields. The fields that were filled with the wrong data are highlighted in red. Please notice that select boxes have to be set again after submitting wrong data.
This page is for submitting the data of your target production. Fill out ''' every '''  field marked with a red star ( ''' required fields ''' ). If you just want to enter the data of '''one''' target do '''not''' fill '''any''' of the fields of '''!target2'''. An error message will appear on top of the page if you submitted data in the wrong format or did not fill all reqired fields. The fields that were filled with the wrong data are highlighted in red.  


== Required fields ==
== Required fields ==
Line 6: Line 6:
Fields that have to be filled in for at least one RNA
Fields that have to be filled in for at least one RNA


* Date, date of the RNA preparation
* Date, date of the target preparation
* Operator, the person who performed the RNA preparation (already filled in)
* Operator, the person who performed the target preparation (already filled in)
* Organism, the organism from which the RNA was extracted (default is "None". Make sure you selected the correct organism)
* Polymer type, whether your target was extracted from a RNA or DNA.
* Tissue and strain, the tissue the RNA was prepared from or the micro-organism strain used to prepare RNA
* Organism, the organism from which the target was extracted (default is "None". Make sure you selected the correct organism)
* Tissue and strain, the tissue the target was prepared from or the micro-organism strain used to prepare the target
* Growth conditions, the biological conditions the tissues were harvested / the strains were grown. The more detailed and informative your description, the better
* Growth conditions, the biological conditions the tissues were harvested / the strains were grown. The more detailed and informative your description, the better
* Volume, the volume of the RNA in ul
* Volume, the total volume of the produced target in ul
* Concentration, the concentration of the RNA in ug/ul
* Concentration, the concentration of the target in ug/ul
* RNA ID, ID of the RNA
* Target ID, ID of the target
* Preparation protocol, select an existing protocol (default is "None") or upload a new one in pdf format
* Preparation protocol, select an existing protocol (default is "None") or upload a new one in pdf format
* Protocol description, only required if a new protocol is uploaded. The description yon enter will become the protocol name
* Protocol description, only required if a new protocol is uploaded. The description yon enter will become the protocol name


  It's also possible to upload images of the RNA on a separation gel. If you do so you have to fill
  It's also possible to upload images of the target on a separation gel. If you do so you have to fill


* µl (RNA1 loaded), how much of RNA1 was loaded to the gel
* µl (!target1 loaded), how much of target1 was loaded to the gel
* gel type, what kind of gel was used  
* gel type, what kind of gel was used  


Line 28: Line 29:
* o.D.260/o.D.280, optical density values
* o.D.260/o.D.280, optical density values
* o.D.260/o.D.230, optical density values
* o.D.260/o.D.230, optical density values
* Remarks, some remarks of your RNA production
* Remarks, some remarks of your target production
* Alterations for the preparation protocol used
* Alterations for the preparation protocol used
* Images of the RNA on a separation gel
* Images of the target on a separation gel

Latest revision as of 07:17, 26 October 2011

This page is for submitting the data of your target production. Fill out every field marked with a red star ( required fields ). If you just want to enter the data of one target do not fill any of the fields of !target2. An error message will appear on top of the page if you submitted data in the wrong format or did not fill all reqired fields. The fields that were filled with the wrong data are highlighted in red.

Required fields

Fields that have to be filled in for at least one RNA

  • Date, date of the target preparation
  • Operator, the person who performed the target preparation (already filled in)
  • Polymer type, whether your target was extracted from a RNA or DNA.
  • Organism, the organism from which the target was extracted (default is "None". Make sure you selected the correct organism)
  • Tissue and strain, the tissue the target was prepared from or the micro-organism strain used to prepare the target
  • Growth conditions, the biological conditions the tissues were harvested / the strains were grown. The more detailed and informative your description, the better
  • Volume, the total volume of the produced target in ul
  • Concentration, the concentration of the target in ug/ul
  • Target ID, ID of the target
  • Preparation protocol, select an existing protocol (default is "None") or upload a new one in pdf format
  • Protocol description, only required if a new protocol is uploaded. The description yon enter will become the protocol name
It's also possible to upload images of the target on a separation gel. If you do so you have to fill
  • µl (!target1 loaded), how much of target1 was loaded to the gel
  • gel type, what kind of gel was used

Optional fields

You may also add some additional data for your RNA production

  • o.D.260/o.D.280, optical density values
  • o.D.260/o.D.230, optical density values
  • Remarks, some remarks of your target production
  • Alterations for the preparation protocol used
  • Images of the target on a separation gel