Target labeling: Difference between revisions

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This form is for submitting data of the labeling targets you extracted from certain RNA. Fill out every  field marked with a red star ( required fields ). If you just want to enter the data of '''one''' target do '''not''' fill '''any''' of the fields of target 2. An error message will appear on top of the page if you submitted data in the wrong format or did not fill in all reqired fields. The fields that were filled in with the wrong data are highlighted in red. Please notice that select boxes have to be set again after submitting wrong data.
This form is for submitting data of the labeling process of your targets. Fill out ''' every ''' field marked with a red star ('''required fields'''). If you just want to enter the data of '''one''' target do '''not''' fill '''any''' of the fields of '''target2'''. An error message will appear on top of the page either if you have submitted data in the wrong format or did not fill out all required fields. The fields that were filled out with the wrong data are highlighted in red.  


== Required fields ==
== Required fields ==


Fields that have to be filled for at least one labeling target
The following fields have to filled out for at least on of the labeling targets:


* Date, date of the RNA preparation
* '''Date''': date of the labeled target preparation
* Operator, the operator who conducts the labelling process (already filled in)
* '''Operator''': the operator who conducts the labeling process (already filled out, only change this if the person who performed the labeling is not the same who is currently logged in!)
* RNA-ID, the RNA from which the target was created (default is "None". Make sure you selected the correct RNA-ID)
* '''Target-ID''': the unique target identification from which the target was created (default is "None". Make sure you selected the correct target-ID)
* Amount, the amount of RNA used for the labelling in ug
* '''Amount''': the amount of target used for the labeling in µg
* Dye, the dye used for labelling (default is "None". Make sure you selected the correct dye)
* '''Dye''': the dye used for labeling (default is "None". Make sure you selected the correct dye)
* Hybridization target ID, ID of the labeled target
* '''Labeled target ID''': ID of the labeled target
* Labeling protocol, select an existing protocol (default is "None") or upload a new one in pdf format
* '''Labeling protocol''': select an existing protocol (default is "None") or upload a new one in .pdf format
* Protocol description, only required if a new protocol is uploaded
* '''Protocol description''': only required if a new protocol is uploaded
* '''Abstract of the protocol''': only required if a new protocol is uploaded. The abstract is needed for the MAGE-ML export in EMMA. Please fill in a short abstract of the protocol. The abstract should embody all important information of the protocol.
<br>
It's also possible to upload images of the labeled target on a separation gel. If you do so you have to fill out these fields:


It's also possible to upload images of the labeling target on a separation gel. If you do so you have to fill in
* '''µl (labeled-target1 loaded)''': how much of the labeled-target was loaded to the gel
* '''Gel type''': what kind of gel was used


* µl (RNA1 loaded), how much of the RNA was loaded to the gel
== Optional fields ==
* gel type, what kind of gel was used


=== Optional fields ===
You may also add some additional data for your labeling process:


You may also add some additional data for your labeling process
* '''Volume''': the volume of the probe used in ul
 
* '''Remarks''': some remarks of your labeling process
* Volume, the volume of the probe used in ul
* '''Gel images''': you can upload a photo of the labeling on a separation gel
* Remarks, some remarks of your labeling process
* Alterations for the labeling protocol
* Images of the labeling on a separation gel

Latest revision as of 07:18, 26 October 2011

This form is for submitting data of the labeling process of your targets. Fill out every field marked with a red star (required fields). If you just want to enter the data of one target do not fill any of the fields of target2. An error message will appear on top of the page either if you have submitted data in the wrong format or did not fill out all required fields. The fields that were filled out with the wrong data are highlighted in red.

Required fields

The following fields have to filled out for at least on of the labeling targets:

  • Date: date of the labeled target preparation
  • Operator: the operator who conducts the labeling process (already filled out, only change this if the person who performed the labeling is not the same who is currently logged in!)
  • Target-ID: the unique target identification from which the target was created (default is "None". Make sure you selected the correct target-ID)
  • Amount: the amount of target used for the labeling in µg
  • Dye: the dye used for labeling (default is "None". Make sure you selected the correct dye)
  • Labeled target ID: ID of the labeled target
  • Labeling protocol: select an existing protocol (default is "None") or upload a new one in .pdf format
  • Protocol description: only required if a new protocol is uploaded
  • Abstract of the protocol: only required if a new protocol is uploaded. The abstract is needed for the MAGE-ML export in EMMA. Please fill in a short abstract of the protocol. The abstract should embody all important information of the protocol.


It's also possible to upload images of the labeled target on a separation gel. If you do so you have to fill out these fields:
  • µl (labeled-target1 loaded): how much of the labeled-target was loaded to the gel
  • Gel type: what kind of gel was used

Optional fields

You may also add some additional data for your labeling process:

  • Volume: the volume of the probe used in ul
  • Remarks: some remarks of your labeling process
  • Gel images: you can upload a photo of the labeling on a separation gel