RNA description: Difference between revisions

From BRF-Software
Jump to navigation Jump to search
imported>TimKahlke
No edit summary
m (11 revisions)
 
(7 intermediate revisions by 2 users not shown)
Line 1: Line 1:
__NOTOC__
__NOTOC__
= RNA description =
This page is for submitting the data of your RNA production. Fill out ''' every '''  field marked with a red star ( ''' required fields ''' ). If you just want to enter the data of '''one''' RNA do '''not''' fill '''any''' of the fields of '''RNA2'''. An error message will appear on top of the page if you submitted data in the wrong format or did not fill all reqired fields. The fields that were filled with the wrong data are highlighted in red. Please notice that select boxes have to be set again after submitting wrong data.


Here you store the data of your RNA production. Fill out ''' every '''  field marked with a red star ( ''' required fields ''' ).If you just want to enter the data of '''one''' RNA do '''not''' fill '''any''' of the fields of '''RNA2'''.
== Required fields ==


== Requiered fields ==
Fields that have to be filled in for at least one RNA


Fields that have to be filled for at least one RNAs
* Date, date of the RNA preparation
 
* Operator, the person who performed the RNA preparation (already filled in)
* Date, date of the RNA preparation)
* Organism, the organism from which the RNA was extracted (default is "None". Make sure you selected the correct organism)
* Operator, the person who performed the purification (is already filled)
* Tissue and strain, the tissue the RNA was prepared from or the micro-organism strain used to prepare RNA
* Organism, the organism from which the RNA is extracted(Default is "None".Make sure you selected the wright organism)
* Growth conditions, the biological conditions the tissues were harvested / the strains were grown. The more detailed and informative your description, the better
* Tissue and strain
* Volume, the volume of the RNA in ul
* Growth conditions
* Concentration, the concentration of the RNA in ug/ul
* Volume, the volume used in ul
* RNA ID, ID of the RNA
* Concentration, the concentration in ug/ul
* Preparation protocol, select an existing protocol (default is "None") or upload a new one in pdf format
* RNA ID, label ID of the RNA
* Protocol description, only required if a new protocol is uploaded. The description yon enter will become the protocol name
* Preparation protocol, select an existing protocol or upload a new one
* Protocol description, only required if a new protocol is uploaded


  It's also possible to upload images of the RNA on a separation gel. If you do so you have to fill
  It's also possible to upload images of the RNA on a separation gel. If you do so you have to fill


* µl (RNA1 loaded), how much of the RNA was loaded to the gel
* µl (RNA1 loaded), how much of RNA1 was loaded to the gel
* gel type, what kind of gel was used  
* gel type, what kind of gel was used  


=== Optional fields ===
=== Optional fields ===


You can also add some additional data for your RNA production
You may also add some additional data for your RNA production


* o.D.260/o.D.280
* o.D.260/o.D.280, optical density values
* o.D.260/o.D.230
* o.D.260/o.D.230, optical density values
* Remarks, some remarks of your RNA production
* Remarks, some remarks of your RNA production
* Alterations for the preparation protocol
* Alterations for the preparation protocol used
* Images of the RNA on a separation gel
* Images of the RNA on a separation gel

Latest revision as of 07:17, 26 October 2011

This page is for submitting the data of your RNA production. Fill out every field marked with a red star ( required fields ). If you just want to enter the data of one RNA do not fill any of the fields of RNA2. An error message will appear on top of the page if you submitted data in the wrong format or did not fill all reqired fields. The fields that were filled with the wrong data are highlighted in red. Please notice that select boxes have to be set again after submitting wrong data.

Required fields

Fields that have to be filled in for at least one RNA

  • Date, date of the RNA preparation
  • Operator, the person who performed the RNA preparation (already filled in)
  • Organism, the organism from which the RNA was extracted (default is "None". Make sure you selected the correct organism)
  • Tissue and strain, the tissue the RNA was prepared from or the micro-organism strain used to prepare RNA
  • Growth conditions, the biological conditions the tissues were harvested / the strains were grown. The more detailed and informative your description, the better
  • Volume, the volume of the RNA in ul
  • Concentration, the concentration of the RNA in ug/ul
  • RNA ID, ID of the RNA
  • Preparation protocol, select an existing protocol (default is "None") or upload a new one in pdf format
  • Protocol description, only required if a new protocol is uploaded. The description yon enter will become the protocol name
It's also possible to upload images of the RNA on a separation gel. If you do so you have to fill
  • µl (RNA1 loaded), how much of RNA1 was loaded to the gel
  • gel type, what kind of gel was used

Optional fields

You may also add some additional data for your RNA production

  • o.D.260/o.D.280, optical density values
  • o.D.260/o.D.230, optical density values
  • Remarks, some remarks of your RNA production
  • Alterations for the preparation protocol used
  • Images of the RNA on a separation gel