MeltDBWiki/HowTos: Difference between revisions

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== Installing a MeltDB server on Mac OS X ==
== Using the public MeltDB experiments ==


Prerequisities:
* Login to the public MeltDB project using the guest account:
  [http://meltdb.cebitec.uni-bielefeld.de/cgi-bin/meltdb.cgi?login=guest&chksum=h/Zx4JEg6sz0I6hi97ZfsA&project=MeltDB_Public MeltDB guest login]


* xampp (version 0.5) package for Mac OS X
* To analyze the publicly available datasets in MeltDB, please select 'Browse/Analyse an existing Experiment' from the start page.
* R
* XCMS
* metaB


checkout the following modules from the bioinfo CVS repository at [[CeBiTec]]
[[Image:MeltDBWiki$$UploadChromas$startpage.png]]


* MeltDB
* Select the 'Three carbon sources' experiment by clicking on the folder icon to the left.
* MeltDB_web
* projectmanagement


[[Image:MeltDBWiki$$HowTos$experiments.png]]


<pre><nowiki>
* The textual display of the experiment is presented and a number of potential actions together with their short descriptions are listed below.
cd
mkdir Melt
cd Melt
cvs update -dPA MeltDB MeltDB_web projectmanagement
</nowiki></pre>


[[Image:MeltDBWiki$$HowTos$carbon_sources.png]]


* Please select 'Show PCA' to start the PCA analysis on the previously imported peaks found by Xcalibur preprocessing methods.
* Select all chromatograms and a list of metabolites and click the 'Compute PCA' button. If you would like to log-transform the measured and normalized metabolite values, mark the 'log' checkbox.


<pre><nowiki>
[[Image:MeltDBWiki$$HowTos$pca.png]]
su root
cd /Applications/xampp/xamppfiles/lib
chmod 755 perl5
</nowiki></pre>
 
 
=== Install additional perl Modules ===
 
 
<pre><nowiki>
cd /Applications/xampp/xamppfiles/bin/
./cpan CGI::Session::MySQL Digest::MD5 DB_File::Lock Fcntl Crypt::GeneratePassword DBI Config::IniFiles HTML::Template CGI::Ajax
</nowiki></pre>
 
 
=== Create user_conf.sh at MeltDB_web/share/www ===
 
 
<pre><nowiki>
PORT=60086
INSTALLDIR=/Users/<your_login>/Melt/
APACHEDIR=/Applications/xampp/xamppfiles/
</nowiki></pre>
 
 
=== Start the MeltDB webserver ===

Latest revision as of 16:12, 31 October 2011

Using the public MeltDB experiments

  • Login to the public MeltDB project using the guest account:
  MeltDB guest login
  • To analyze the publicly available datasets in MeltDB, please select 'Browse/Analyse an existing Experiment' from the start page.
MeltDBWiki$$UploadChromas$startpage.png
  • Select the 'Three carbon sources' experiment by clicking on the folder icon to the left.
MeltDBWiki$$HowTos$experiments.png
  • The textual display of the experiment is presented and a number of potential actions together with their short descriptions are listed below.
MeltDBWiki$$HowTos$carbon sources.png
  • Please select 'Show PCA' to start the PCA analysis on the previously imported peaks found by Xcalibur preprocessing methods.
  • Select all chromatograms and a list of metabolites and click the 'Compute PCA' button. If you would like to log-transform the measured and normalized metabolite values, mark the 'log' checkbox.
MeltDBWiki$$HowTos$pca.png