GenDBWiki/WebDocumentation/DialogWindows/OrthologViewer: Difference between revisions

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The Ortholog Viewer is a tool that shows ortholog CDS in similar genomes. The orthologs have to be computed before.<br>
The Ortholog Viewer is a tool that shows ortholog CDS in similar genomes. The orthologs have to be computed before.<br>
<br>
<br>
What should be done to use this tool?<br>
What should be done to use this tool? (Points 1-3 are already done, if you prepared the project for the [[GenDBWiki/WebDocumentation/DialogWindows/ComparativeAnnotator|Comparative Annotator]]<br>
# create Bidirectional-Blast-Tools in the project with: ''create_genome_compare_tools.pl''<br>
# create Bidirectional-Blast-Tools in the project with: ''create_genome_compare_tools.pl''<br>
# Run the Tool for each Contig-combination with: ''submit_job.pl''<br>
# Run the Tool for each Contig-combination with: ''submit_job.pl''<br>
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# Use the tool<br>
# Use the tool<br>
<br>
<br>
== Contig View ==
 
This is how the window looks like after opening the Ortholog Viewer:<br>


[[Image:GenDBWiki$$WebDocumentation$$DialogWindows$$OrthologViewer$ortholog_viewer_1.gif]]
[[Image:GenDBWiki$$WebDocumentation$$DialogWindows$$OrthologViewer$ortholog_viewer_1.gif]]


On the right side of the image you can do the following:
Now you can do the following:
* [[#AnnotateReference|Annotate the Reference]]
* [[#BrowseOrthologs|Browse through the genome]]
* [[#CopyAnnotation|Copy Annotation]]  
* [[#SelectCDS|Select a CDS]]
* [[#ViewMultipleAlignment|View multiple alignment]]
 
[[Anchor(Browse Orthologs)]]
==== Browse through the genome ====
You can browse through the genome, using the scrollbar. When moving the mouse over a CDS-box, its orthologs are highlighted yellow:<br>
[[Image:GenDBWiki$$WebDocumentation$$DialogWindows$$OrthologViewer$ortholog_viewer_2.gif]]
 
When you find an interesting CDS you may examine it further by selecting it.<br>


<span id="AnnotateReference"></span>
<span id="SelectCDS"></span>
==== Annotate the reference ====
==== Select a CDS ====
By clicking a CDS-box, one CDS is selected (red) and its orthologs are marked orange. Information about all highlighted CDS are shown below the image. Loading the information may take some seconds. Please be patient. <br>


You can select one CDS as reference and annotate it (or check if the annotation is correct), using the [[GenDBWiki/WebDocumentation/DialogWindows/AnnotationDialog|Annotation Dialog]].<br>
[[Image:GenDBWiki$$WebDocumentation$$DialogWindows$$OrthologViewer$ortholog_viewer_3.gif]]
- select the desired CDS as '''Reference'''<br>
- click the '''"Annotate Reference"''' - button<br>
- do your annotation<br>


[[Image:GenDBWiki$$WebDocumentation$$DialogWindows$$OrthologViewer$compAnno_annotateReference.gif]]
After selecting a CDS you get a menu:


<span id="CopyAnnotation"></span>
[[Image:GenDBWiki$$WebDocumentation$$DialogWindows$$OrthologViewer$ortholog_viewer_4.gif]]
==== Copy Annotation ====


You can copy the annotation from one CDS to others.<br>
You can open the [[GenDBWiki/WebDocumentation/DialogWindows/ComparativeAnnotator|Comparative Annotator]] or select the chosen CDS in the Main-Window.

Latest revision as of 07:14, 26 October 2011

Ortholog Viewer

The Ortholog Viewer is a tool that shows ortholog CDS in similar genomes. The orthologs have to be computed before.

What should be done to use this tool? (Points 1-3 are already done, if you prepared the project for the Comparative Annotator

  1. create Bidirectional-Blast-Tools in the project with: create_genome_compare_tools.pl
  2. Run the Tool for each Contig-combination with: submit_job.pl
  3. Run set_biderectional_project_config.pl.
  4. Run compute_orthologs.pl to create ortholog-observations
  5. Use the tool


This is how the window looks like after opening the Ortholog Viewer:

File:GenDBWiki$$WebDocumentation$$DialogWindows$$OrthologViewer$ortholog viewer 1.gif

Now you can do the following:

Anchor(Browse Orthologs)

Browse through the genome

You can browse through the genome, using the scrollbar. When moving the mouse over a CDS-box, its orthologs are highlighted yellow:
File:GenDBWiki$$WebDocumentation$$DialogWindows$$OrthologViewer$ortholog viewer 2.gif

When you find an interesting CDS you may examine it further by selecting it.

Select a CDS

By clicking a CDS-box, one CDS is selected (red) and its orthologs are marked orange. Information about all highlighted CDS are shown below the image. Loading the information may take some seconds. Please be patient.

File:GenDBWiki$$WebDocumentation$$DialogWindows$$OrthologViewer$ortholog viewer 3.gif

After selecting a CDS you get a menu:

File:GenDBWiki$$WebDocumentation$$DialogWindows$$OrthologViewer$ortholog viewer 4.gif

You can open the Comparative Annotator or select the chosen CDS in the Main-Window.