RNA description: Difference between revisions

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Latest revision as of 07:17, 26 October 2011

This page is for submitting the data of your RNA production. Fill out every field marked with a red star ( required fields ). If you just want to enter the data of one RNA do not fill any of the fields of RNA2. An error message will appear on top of the page if you submitted data in the wrong format or did not fill all reqired fields. The fields that were filled with the wrong data are highlighted in red. Please notice that select boxes have to be set again after submitting wrong data.

Required fields

Fields that have to be filled in for at least one RNA

  • Date, date of the RNA preparation
  • Operator, the person who performed the RNA preparation (already filled in)
  • Organism, the organism from which the RNA was extracted (default is "None". Make sure you selected the correct organism)
  • Tissue and strain, the tissue the RNA was prepared from or the micro-organism strain used to prepare RNA
  • Growth conditions, the biological conditions the tissues were harvested / the strains were grown. The more detailed and informative your description, the better
  • Volume, the volume of the RNA in ul
  • Concentration, the concentration of the RNA in ug/ul
  • RNA ID, ID of the RNA
  • Preparation protocol, select an existing protocol (default is "None") or upload a new one in pdf format
  • Protocol description, only required if a new protocol is uploaded. The description yon enter will become the protocol name
It's also possible to upload images of the RNA on a separation gel. If you do so you have to fill
  • µl (RNA1 loaded), how much of RNA1 was loaded to the gel
  • gel type, what kind of gel was used

Optional fields

You may also add some additional data for your RNA production

  • o.D.260/o.D.280, optical density values
  • o.D.260/o.D.230, optical density values
  • Remarks, some remarks of your RNA production
  • Alterations for the preparation protocol used
  • Images of the RNA on a separation gel