ProDBWiki/DeveloperDocumentation/ImportMultiScans

Import Multi Scans

Overview

If you have a gel picture with many protein spots from a tissue, and you want to know what kind of proteins that are, you take a sample from the spots and analyse them with mass spectrometry. As a result you get a file with the peptide masses. Now you have to import the files and connect them with the corresponding gel spot. This is a lot of work, especially if you have a large number of spots/files. Therefore you can use the Multi Files Importer. Here you can import all files from spots from one gel at once. You save all the files and the robotfile from the spot selector in an compressed file. The compressed file has to be a .tgz-, .tar.gz-, .tar- or .zip-file. Also you have to observe that the compressed file has to be from a special form. This tool uploads all files at once and connect them automatically with the right gel spot.

How to use

At first you have to import a gel-picture and select the spots you want to analyse. In the robot/config-file you find the the directories where the ms-files for one spot have to be saved (normally the mass spectrometer does it automatically). Be aware that the config file has to be in a specific format, described below. After you selected the spots and send them into the mass spectrometer you have to create a workstep for ms import and select all samples(spots) that were used by the spot selector.

Formats

Format of the compressed File

Author: Nicole de la Chaux