MeltDBWiki/RIManual: Difference between revisions
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* Select the chromatogram for which you would like to annotate the Retention Indices using e.g. the iMelt overview or the search function. | * Select the chromatogram for which you would like to annotate the Retention Indices using e.g. the iMelt overview or the search function. | ||
[[Image:MeltDBWiki$$RIManual$rimanual.png]] | [[Image:MeltDBWiki$$RIManual$rimanual.png]] | ||
* After you opened your chromatogram, please select 'Annotate retention indices manually' from the action list. | * After you opened your chromatogram, please select 'Annotate retention indices manually' from the action list. | ||
[[Image:MeltDBWiki$$RIManual$riui.png]] | [[Image:MeltDBWiki$$RIManual$riui.png]] | ||
* Select the retention times of the alkane standard peaks that were added to the measurement and move them in ascending order to the dialog box at the left. | * Select the retention times of the alkane standard peaks that were added to the measurement and move them in ascending order to the dialog box at the left. | ||
* For each selected peak, specify the corresponding Retention Index in the right text box. | * For each selected peak, specify the corresponding Retention Index in the right text box. | ||
[[Image:MeltDBWiki$$RIManual$riui2.png]] | [[Image:MeltDBWiki$$RIManual$riui2.png]] | ||
* Click the 'Compute RI' button | |||
=== Identifying Alkane Standards === | |||
* The TIC view of your experiment can help you to identify the Alkane Peaks. The ions with mass-to-charge ratio 57, 71, 85, 99 are typical for the alkane standards. | * The TIC view of your experiment can help you to identify the Alkane Peaks. The ions with mass-to-charge ratio 57, 71, 85, 99 are typical for the alkane standards. | ||
[[Image:MeltDBWiki$$RIManual$ritic.png]] | [[Image:MeltDBWiki$$RIManual$ritic.png]] | ||
Revision as of 13:11, 8 March 2010
Manual Retention Index Annotation
- Login to the your MeltDB project using your authorized account
- MeltDB login
- Select the chromatogram for which you would like to annotate the Retention Indices using e.g. the iMelt overview or the search function.
- After you opened your chromatogram, please select 'Annotate retention indices manually' from the action list.
- Select the retention times of the alkane standard peaks that were added to the measurement and move them in ascending order to the dialog box at the left.
- For each selected peak, specify the corresponding Retention Index in the right text box.
- Click the 'Compute RI' button
Identifying Alkane Standards
- The TIC view of your experiment can help you to identify the Alkane Peaks. The ions with mass-to-charge ratio 57, 71, 85, 99 are typical for the alkane standards.
